"The whole psychedelic scene came from that bus trip," said Kesey's son, Zane, who as a 3-year-old helped paint the bus and waved a tearful goodbye as it drove away.
Now, on the 50th anniversary of the journey, Zane Kesey has launched a campaign on the crowd-funding site Kickstarter, looking for a few good modern-day Pranksters to contribute $200 each for a chance to do it all again, though on a different bus. And without the LSD.
His father, who wrote "One Flew Over the Cuckoo's Nest," had been a guinea pig for government tests of the mind-altering drug and shared the experience at a series of parties at his home in the hills above Stanford University that became known as the Acid Tests.
They featured a local bar band called The Warlocks, which turned into the Grateful Dead. After the trip, the bus became the centerpiece as the Acid Tests went on the road.
After a road trip to New York with his parents and wife, Faye, to see the stage production of "Cuckoo's Nest," Kesey thought it would be fun to get his friends to do another cross-country ride that would serve as the basis for a movie, with LSD at the center, friend Ken Babbs said.
It soon became clear that the family station wagon would not be big enough, so Kesey sent a friend up to San Francisco to check out an ad for an old school bus converted into a camper, with bunks and a kitchen, Babbs said.
"I think it was $1,500," he said.
During the journey, the bus was pulled over by a policeman in California, got bogged down in an Arizona River and lost one of its crew to a bad trip in Texas.
In Louisiana, the Pranksters jammed with a piano player in a New Orleans bar. In New York, they rolled through the streets playing their homemade music and met poet Alan Ginsberg, who took them to a Connecticut estate to meet LSD guru Timothy Leary.
The movie never materialized as the new art form Kesey had envisioned, a victim of the film and audio tapes reproducing at different speeds that couldn't be synchronized until 30 years later with the help of digital technology. Kesey died in 2001.
Texas A&M historian Terry Anderson, author of the book, "The Sixties," said the bus trip was too early to kick off the counterculture, adding that it was overshadowed by the Beatles and the signing of the Civil Rights Act the same year.
But the tapes and film gave author Tom Wolfe the material he needed for his 1968 book, "The Electric Kool-Aid Acid Test," which made the trip a touchstone of the psychedelic era.
Babbs said Kesey himself never claimed to have started the psychedelic era, but he was happy to ride the wave. "We were too young to be beats, and too old to be hippies," he said. "We were really our own thing."
Embarking at the end of July, the anniversary trip is making no attempt to recreate the old one, and follows a more northerly route, visiting a series of arts and music festivals.
People who want a chance to get on the bus must invest $200, and pass a series of tests, answering questions such as whether they like movies about gladiators. The chosen will get a token to board the bus for a leg, and join in making a new movie.
Though not even born when the original bus embarked on June 17, 1964, Joshua Priest, 26, and Andrea Castillo, 21, of Menisee, Calif., are determined to get onboard.
Grateful Dead fans who learned about the bus trip by reading Wolfe's book, they learned about the anniversary expedition on Facebook and raised $400 with a garage sale. Castillo, a graphic arts student, created a series of drawings answering the questions.
"That's what life is about: taking chances and having fun," she said.