Putin's order appears to show that Russia, although increasingly suffering the effects of Western sanctions, is disinclined to back down on Ukraine. It follows the latest round of sanctions against Russia imposed by the EU last week, which for the first time targeted entire sectors of the Russian economy.
The U.S. and the EU have accused Russia, which annexed Ukraine's Crimean Peninsula in March, of fomenting tensions in eastern Ukraine by supplying arms and expertise to a pro-Moscow insurgency, and have imposed asset freezes and loan bans on a score of individuals and companies. Russia denies such allegations.
White House spokeswoman Laura Lucas Magnuson decried the import ban, saying "Retaliating against Western companies or countries will deepen Russia's international isolation, causing further damage to its own economy."
Russia depends heavily on imported foodstuffs most of it from the West particularly in the largest and most prosperous cities such as Moscow. Food and agricultural imports from the U.S. amounted to $1.3 billion last year, according to the U.S. Department of Agriculture, and in 2013 the EU's agricultural exports to Russia totaled 11.8 billion euros ($15.8 billion).
U.S. poultry exports make up the largest portion of the total, with a little more than $300 million in exports to Russia last year. According to the National Chicken Council, Russia is the second-leading market for U.S. chicken in terms of volume and the third in terms of value.
Soybeans, tree nuts, tobacco and prepared foods were other U.S. exports.
Putin's order says the limits are being imposed "with the goal of guaranteeing the security of the Russian Federation" and calls for undertaking measures to guard against quick price hikes.
As tensions over Ukraine rise, a respected newspaper this week cited unnamed sources as saying Russia is considering closing its airspace to European carriers flying to Asia. The report sent the stocks of some airlines sharply lower.
Russian Foreign Minister Sergey Lavrov on Wednesday said he wouldn't comment on "rumors" of airspace being closed, but said "our Western partners should think about their companies and their citizens," the Interfax news agency reported.
Russia last week banned the import of apples and some other fruits from Poland, saying this was because of sanitary concerns, but raising speculation that the move was in retaliation for Poland's support of the Ukrainian authorities.
Josh Lederman and Mary Clare Jalonick in Washington contributed to this report.